Zone Centrifugation in a Cesium Chloride Density Gradient Caused by Temperature Change
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چکیده
منابع مشابه
Equilibrium Density Gradient Centrifugation in Cesium Chloride Solutions Developed by Matthew Meselson and Franklin Stahl
Matthew Meselson, Franklin Stahl, and Jerome Vinograd, developed cesium chloride, or CsCl, density gradient centrifugation in the 1950s at the California Institute of Technology [5], or Caltech, in Pasadena, California. Density gradient centrifugation enables scientists to separate substances based on size, shape, and density. Meselson and Stahl invented a specific type of density gradient cent...
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This method is used to extract high molecular weight genomic DNA from planktonic biomass concentrated on 0.22 microM Sterivex filters that have been treated with storage/lysis buffer and archived at -80 degrees C, and to purify this DNA using a cesium chloride density gradient. The protocol begins with two one-hour incubation steps to liberate DNA from cells and remove RNA. Next, a series of Ph...
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1. The mucoprotein from pig gastric mucus has been purified by equilibrium centrifugation in a CsCl gradient. 2. This procedure removes the non-covalently bound protein, which is closely associated with the mucoprotein and not easily removed from it by gel filtration. 3. The purified mucoprotein is separable by gel filtration into a high-molecular-weight mucoprotein A (mol.wt. 2.3x10(6)) and a ...
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Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1965
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(65)86761-3